rabbit polyclonal igg anti aromatase  (Novus Biologicals)

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Primary and secondary antisera used for Western blot, IFL, and IHC.

Article Snippet: Aromatase (Cyp19) anti-rabbit polyclonal IgG (E-AB-64300, Elabscience: Houston, TX, USA) , 1:2000.

Techniques: Western Blot, Plasmid Preparation

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Modulation of Kiss1R, Kiss1, Cyp19, GnRH , and miR expression in mediobasal hypothalamus homogenates. Representative Western blots ( n = 4) for Kiss1R, Kiss1, and Cyp19 (A) and normalization of Kiss1R (B) , Kiss1 (C) , and Cyp19 signals (D) carried out against α-tubulin. Quantitative expression ( n = 6) of GnRH (E) and miR-132-3p , Mir-145-5p , let-7a-5p , and let-7b-5p (F–I) carried out by qPCR. Data are expressed as protein OD/tubulin OD ± standard deviation or normalized fold expression (n.f.e.) ± SEM against the housekeeping genes HPRT / β-actin / U6 and the control group used as reference sample. C, control group (placebo injected animals); KP, animals injected with Kp10; AEA, animals injected with AEA; SR + AEA (SA), animals first received SR141716A and 30 min later received AEA treatment. ** p < 0.01, * p < 0.05 vs. the control group.

Article Snippet: Aromatase (Cyp19) anti-rabbit polyclonal IgG (E-AB-64300, Elabscience: Houston, TX, USA) , 1:2000.

Techniques: Expressing, Western Blot, Standard Deviation, Control, Injection

Journal: Frontiers in Endocrinology

Article Title: The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction in vivo

doi: 10.3389/fendo.2023.1269334

Figure Lengend Snippet: Modulation of the intratesticular markers of steroidogenesis and GnRH . Representative Western blots for 3βHsd and Cyp19 (A) and normalization of 3βHsd (B) and Cyp19 (C) signals carried out against α-tubulin. Quantitative expression of 3βHsd , Cyp19 , and GnRH mRNA carried out by qPCR (D) . Data are representative of n = 6 different animals and are expressed as protein OD/tubulin OD ± standard deviation or normalized fold expression (n.f.e.) ± SEM against the housekeeping genes HPRT / β-actin and the control group used as a reference sample. C, control group (placebo-injected animals); KP, animals injected with Kp10; * p < 0.05.

Article Snippet: Aromatase (Cyp19) anti-rabbit polyclonal IgG (E-AB-64300, Elabscience: Houston, TX, USA) , 1:2000.

Techniques: Western Blot, Expressing, Standard Deviation, Control, Injection

Journal: BioMed Research International

Article Title: Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits

doi: 10.1155/2017/2089645

Figure Lengend Snippet: Primary and secondary antibodies and normal sera used in the present study.

Article Snippet: Rabbit polyclonal IgG anti-aromatase , 1 : 500 , NB-200-1596 , Novus Biologicals.

Techniques: